Cell Culture Products for Microscopy
With its advantages for molecular selectivity and capability of live observation, fluorescence microscopy currently is among the most widely used approaches for high resolution, non-invasive imaging of living cells. Depending on the complexity of live cell imaging experiments and the requirements of the corresponding microscope, the requests for the utilised disposables can be as comprehensive. Greiner Bio One’s CELLview imaging consumables are tailored solutions to provide optimal basic settings for your microscopic experiment:
CELLview products combine the convenience of a plastic disposable with the high optical quality of a 0.17 mm thin cover glass bottom, providing superior high-resolution microscopic images of in-vitro cultures. The specific design with the embedded cover glass bottom guarantees a single-plane, flat bottom with a consistent working distance, maximal planarity and optimal thermal conductivity in heated platforms. Glass bottom microplates feature excellent intra- and inter-well flatness due to the rigidity of the glass bottom. As microscope objectives are adapted to the bottom thickness and refractive index of cover glass, generally the best optical quality with highest resolution can be achieved using glass bottom disposables.
Glass bottom features:
Beside visual requirements, a glass bottom should also meet cell culture demands and facilitate successful cultivation of various cell types. Due to the general hydrophobicity of glass, only a minor proportion of cells can adhere to it. Therefore these cells are often only loosely attached, do not display their cell-specific morphology and can get lost during staining and washing steps. Furthermore live cell analysis of such ailing cells might not reveal their realistic in-vivo response. To guarantee optimal cell culture conditions during cultivation and microscopic analysis, the CELLview cell culture disposables are provided with two surfaces: Tissue culture treated (TC) for propagation of standard cell lines and Advanced TC surface facilitating in particular cultivation of sensitive and primary cells. The non-biological surface optimisation leads to consistent and homogenous cell attachment reducing cell loss during transfection or staining procedures making previously required protein coatings of glass surfaces dispensable.
