Coagulation Sodium Citrate / CTAD
Sodium citrate is the standard anticoagulant used for coagulation test tubes. The concentration is either 0.109 mol/l (3.2%) or 0.129 mol/l (3.8%).
- Platelet rich plasma for thrombocyte function samples.
- Platelet poor plasma for the predominant number of plasmatic coagulation tests.These two test materials are unsuitable for freezing.
- Platelet free plasma has the same use as platelet poor plasma; as there are almost no thrombocytes contained in the sample, it can be used when freezing is intended.
In addition to buffered citrate solution VACUETTE® CTAD Tubes contain theophylline, adenosine and dipyridamole.
9NC is the letter code for sodium citrate for coagulation tubes and it means 1 part sodium citrate to 9 parts blood.
Collection of blood into CTAD Tubes is recommended for all tests in which it is important to avoid release of platelet factors between collection of blood and performance of the test.
The outside tube made of PET (polyethylenetherephthalate) plastic is optimal for the maintaining the vacuum. There is no special packaging necessary. The inner tube made of PP (polypropylene) functions to prevent evaporation of liquids, and thus the sodium-citrate volume remains constant. There is minimal surface activation in VACUETTE® Sandwich tubes because of the inner tube which is made out of PP, this is the most suitable surface known for coagulation tests.
Hemolyzed specimens should not be processed since there could be activation of the clotting factors. Lipaemic or icteric specimens may also interfere with the instrumentation's optical system affecting the coagulation specimen result.
There have been recent articles indicating that drawing a discard tube is not necessary before drawing the sodium citrate tube. In the CLSI coagulation document (H21-A5) it is still recommended that the citrate tube is the second or third tube drawn. However, in the CLSI guideline for Venipuncture Collection (H3-A6) it states that for routine PT and APTT, the first tube drawn may be used for testing.It is also important to ensure a discard tube is drawn when a blood collection set is used and only a citrate tube is ordered, as the tubing may contain up to 0.5 mL of air that will be drawn into the tube and displace blood volume.
GBO tubes should be stored at 4-25°'C (39-77°F).
VACUETTE® Tubes are irradiated to achieve sterility. These tubes are sterile on the interior only. We cannot recommend re-sterilizing the tubes, primarily due to pressure changes that take place during the re-sterilization cycle. Our tubes are under a specific negative pressure. During the re-sterilization cycle this negative pressure may be disrupted and therefore the tubes may not draw the proper blood volume.
No, GBO does not claim that any of the tubes are endotoxin free.
|Tube Type||Recommended g-force relative centrifugal force (rcf)||Time (min)|
|VACUETTE ® Serum Tubes / with Sep / with Beads||1800 - 2200 g||10-15|
|VACUETTE ® EDTA Tubes / with Sep||1800 - 2200 g||10-15|
|VACUETTE ® Heparin Plasma Tubes / with Sep||1800 - 2200 g||10-15|
|VACUETTE ® Glucose Tubes||1800 - 2200 g||10-15|
|VACUETTE ® Homocystein Detection Tubes||2000 - 2200 g||10|
|VACUETTE ® Coagulation Tubes|
|- Platelet tests (PRP)||150 g||5|
|- Routine tests (PPP)||1500 - 2000 g||10|
|- Preparation for deep freeze plasma (PFP)||2500 - 3000 g||20|
VACUETTE® Blood Collection Tubes are made from a special formulation of the plastic, polyethylenetherephthalate (PET). This special formulation is extremely strong, dimensionally stable, and resistant to chemicals and breakage.
The expiry date indicates the shelf life of the product as determined by functional testing. To ensure accurate draw and test reliability, tubes should not be used after the expiry date. Expiry dates (last day of the month indicated) are printed on every VACUETTE® Blood Collection Tube and packaging.
Yes, VACUETTE® Blood Collection Tubes have a sterile interior.