3D cell culture becomes a routine tool that plays a vital role in life sciences, especially in cancer research, drug discovery and 
toxicology. The approaches are still relatively new despite their early impact.There is still ambiguity in the robustness of culturing techniques, the readiness of the supply chain, and even the use of terminology depicting 3D morphology. The value of 3D cell cultures is defined by the increased dimensionality and access to contact between cells to generate a phenotype predictive of in vivo biology but  performed in vitro. Cell-cell interaction is fundamental, independent of whether 3D structures are called spheroids, organoids, assembloids, or tumoroids, and of course, a phenotype is agnostic to terminology.

Sub Categories

Why 3D Cell Culture?

In preclinical drug discovery validation processes, monolayer cell cultures are still predominant. Nevertheless, 2D cultures can only mimic the conditions of physiological tissue to a limited extent, whereas cells in vivo are able to interact in a three-dimensional network. Therefore, results generated from 2D cultures may often be of limited relevance for clinical effectiveness and may contribute to high attrition rates in the drug development process. The employment of spheroid cultures is regarded as a better rational to develop more predictive in-vitro screening assays for preclinical drug development, especially in cancer research.

In spheroid cultures, cells grow in a three-dimensional system with zones of cellular heterogeneity and nutrient and oxygen gradients, to more closely reflect the in-vivo tumor microenvironment. Comparisons of spheroid cultures and 2D monolayer cultures showed functional differences in tumor cell lines, e.g. alterations in protein expression, phosphorylation patterns and responsiveness to inhibitor molecules.

Advantages of 3D cell culture

  • Mimics in vivo physiological conditions by replicating native tissue microenvironment
  • In vitro with in vivo cell-cell interactions by promoting cell-cell contact and interaction with ECM for predictive drug response
  • Co-culture of several cell types and possibility of human cell usage
  • No ethical difficulties compared to animal usage - Replacement, Reduction and Refinement (3Rs)

3D cell culture solutions

CELLSTAR® cell-repellent surfaces

The CELLSTAR® cell culture vessels with cell-repellent surfaces prevent cell adherence to promote the spontaneous formation of three-dimensional spheroids and as an essential accessory for the magnetic 3D cell culture technology (m3D)

Magnetic 3D cell culture technology (m3D)

The magnetic 3D cell culture technology (m3D) creates 3D cell culture structures by magnetizing cells and using magnet forces.

ThinCert® cell culture inserts

ThinCert® cell culture inserts to mimic a variety of in vivo situations by the two-compartment system.

Explore the world of 3D cell culture


3D Cell Culture
Brochure / PDF, 817 KB
IFU_655846_96-Well BiO Assay Kit
IFU Instructions for Use / PDF, 1 MB / 655846
IFU_781846_384-Well BiO Assay Kit
IFU Instructions for Use / PDF, 205 KB / 781846
IFU 96-Well Bioprinting Kit
IFU Instructions for Use / PDF, 9 MB / 655840, 655841
Clinically relevant inflammatory breast cancer patientderived xenograft–derived ex vivo model for evaluation of tumor-specific therapies
Scientific Publications / PDF, 1 MB
Advanced Development of Primary Pancreatic Organoid Tumor Models for High-Throughput Phenotypic Drug Screening
Scientific Publications / PDF, 1 MB
Publications 3D Cell Culture
Flyer / PDF, 1 MB
IFU_662840_24-Well Bio-Assembler Kit
IFU Instructions for Use / PDF, 9 MB / 662840
CELLSTAR® microplates with cell-repellent surface as platform for BIOMIMESYS®
Scientific Publications / PDF, 318 KB
Forum No 17: CELLSTAR® Cell Culture Vessels with Cell-Repellent Surface
Scientific Publications / PDF, 620 KB
Magnetic 3D-Cell Culture Transfer with Multi-MagPen
Flyer / PDF, 672 KB
m3D White Paper Biocompatibility Nanoshuttle
White Paper / PDF, 2 MB
Magnetic 3D Cell Culture Onepager Biomaterials
Scientific Publications / PDF, 974 KB
IFU 24 Well Multi-MagPen
IFU Instructions for Use / PDF, 1 MB
IFU 96 Well Multi-MagPen
IFU Instructions for Use / PDF, 1 MB
F074093_White Paper GBO PerkinElmer
White Paper / PDF, 822 KB

Please contact us if you have any questions regarding our innovative 3D Cell Culture products!

Glauco Souza, PH.D.

Director of Global Business Development & Innovation 3D Cell Culture

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