CarnoRapid™ – Rapid detection of 8 animal species in food products
- INNOVATIVE AND EFFECTIV
- Colorimetric detection of 8 species on a lateral-flow DNA chip
- 1 multiplex PCR reaction for the detection of 8 species
- No read-out equipment necessary
- SIMULTANEOUSLY DETECTED SPECIES
- BROAD SAMPLE SPECTRUM
- raw, cooked or fully preserved food
- FAST EXTRACTION INCLUDED
- 6 steps, 5 min hands-on time, 30 min incubation time
- minimal hands-on time
- minimal lab equipment
- PCR control
- limit of detection(w/w) 0.01-1%
The horse meat scandal in 2013 highlighted the potential for the abuse of the labelling of animal meat products. False declarations of meat food products lead to the compulsory testing of the origin of food products down to the species level. The scandal highlighted the fact that food could have been falsely labelled for a number of years because the analysis conducted failed to identify a sufficient number of alternative species. The CarnoRapid™ Test allows to test a wider range of animal species in combination with a minimized workload and thus can help to optimize the control of food of animal origin in the future.
The innovative CarnoRapid™ Test lateral flow DNA chip simultaneously identifies 8 animal species in a single analysis with a minimum amount of hands-on time and equipment. The CarnoRapid™ Test is a lateral-flow DNA chip for the qualitative simultaneous detection of eight animal species in raw, cooked or fully preserved food products.
The DNA is extracted by the Fast Extraction procedure. All components for the Fast Extraction procedure are already included in the CarnoRapid™ Test kit. The protocol is exceptionally quicking and user-friendly: only 6 working steps, 5 minutes of hands-on time and 30 minutes incubation time. Minimal lab equipment is required, however an equipment starter package is available, if necessary. After extraction the species-specific DNA of all 8 animal species present in the sample is amplified in a PCR reaction. The PCR reaction can be performed on an easy to operate, slim PCR-Cycler which has a touch screen and a small footprint.
Species differentiation is accomplished by the hybridization and colorimetric detection of PCR products on the CarnoRapid™ Test chip. The analysis procedure on the chip takes only 10 min. A positive result is indicated by a visual band on the lateral-flow chip for each detected species. Each chip contains a PCR control making the CarnoRapid™ Test a robust and reliable detection system.