If you are looking for the optimal cell culture surface for your assay, explore the new CELLCOAT® MultiCoat Test Plate which allows the rapid and easy testing of a variety of biological coatings simultaneously.
Take advantage of the choice of 11 different peptide and protein coatings on a single microplate to find the appropriate in-vivo mimicking environment for your cells. Choose between our 96 well or 384 well SCREENSTAR plates which offer optimum image quality thanks to their cycloolefin bottom with glass-like optical properties and planarity.
By choosing the optimal protein-coated surface for your cells, improve cell attachment, spreading, distribution and growth or modulate differentiation and protein expression.
Poly-D-Lysine (PDL) & Poly-L-Lysine (PLL) are synthetic poly-peptides commonly used for cell culture coatings, providing a highly positive charged surface that enhances cell adhesion. PDL coatings are more durable based on their resistance against proteases released by certain cell types.
Collagen Type I (Col. Type I) is a major component of the extracellular matrix (ECM) of numerous cell types. Collagen coatings help mimic the in-vivo microenvironment and promote cell adhesion, proliferation and differentiation of mesenchymal and connective tissue-derived cells.
Fibronectin is a glycoprotein that connects membrane-spanning receptors (“integrins”) to extracellular matrix proteins like collagens and heparan sulfate proteoglycans. Fibronectin-binding integrin receptors are present in cell membranes of numerous cell types making fibronectin coatings a preferred choice to facilitate fast, strong attachment, spreading and growth of many cell types.
Laminin is a glycoprotein and major component of the basement membrane. It binds to integrin cell surface receptors in the cell membrane and to type IV collagen, fibronectin and proteoglycans in the extracellular matrix. As a vital support for scaffolding the basal lamina, Laminin coatings promote structural integrity, cell adhesion, migration and tissue organisation, and are commonly used for culturing neurons, muscle cells, epithelial and endothelial cells.
Basement membrane extract (BME) is derived from Engelbreth-Holm-Swarm mouse sarcoma, composed predominantly of laminin, type IV collagen and heparan sulfate proteoglycan. It mimics the basement membrane extracellular environment in many tissues and is used as a “thin coating” for cell adhesion and support of cell-specific phenotype in complex 2D-cell models.
Use our MultiCoat Test Plate and rapidly test a variety of coatings at once to find the appropriate biological environment for your cells.
As a further orientation use our surface selection guide:
| Cell type | PDL & PLL | Collagen I | Fibronectin | Laminin | PDL/COL | COL/FN | PDL/LAM | BME |
|---|---|---|---|---|---|---|---|---|
| Cardiomyocytes | x | x | x | x | x | x | x | x |
| Endothelial cells | x | x | x | x | x | x | ||
| Epithelial cells | x | x | x | x | x | |||
| Fibroblasts | x | x | x | x | x | |||
| Hepatocytes | x | x | x | x | x | x | x | |
| Mammary cells | x | x | x | x | x | |||
| Macrophages | x | x | x | x | ||||
| Monocytes | x | x | x | x | x | |||
| Myoblasts | x | x | x | x | x | |||
| Neuronal cells | x | x | x | x | ||||
| Smooth muscle cells | x | x | x | x | x | x | x | x |
SCREENSTAR MICROPLATE, 96 WELL, CYCLOOLEFIN FILM BOTTOM, BLACK, CELLCOAT®, MULTICOAT, LID, 2 PCS./BAG
SCREENSTAR MICROPLATE, 384 WELL, CYCLOOLEFIN FILM BOTTOM, BLACK, CELLCOAT® BASEMENT MEMBRANE EXTRACT, LID, 5 PCS./BAG
SCREENSTAR MICROPLATE, 384 WELL, CYCLOOLEFIN FILM BOTTOM, BLACK, CELLCOAT®, MULTICOAT, LID, 2 PCS./BAG
