Venous Blood Collection
Sodium Citrate is the standard anticoagulant used for coagulation test tubes. The concentration is 0.109 mol/l (3.2%).
In addition to buffered citrate solution, VACUETTE® CTAD Tubes contain theophylline, adenosine and dipyridamole.
9NC is the letter code for sodium citrate for coagulation tubes and it indicates there is a ratio of 1 part sodium citrate to 9 parts blood.
Collection of blood into CTAD Tubes is recommended for all tests in which it is important to avoid release of platelet factors between collection of blood and performance of the test.
The outside tube made of PET (polyethylenetherephthalate) plastic is optimal for the maintaining the vacuum. There is no special packaging necessary. The inner tube, made of PP (polypropylene), functions to prevent evaporation of liquids, and thus the sodium-citrate volume remains constant. There is minimal surface activation in VACUETTE® sandwich tubes because of the inner tube, which is made out of PP, this is the most suitable surface known for coagulation tests.
Yes, the sodium citrate tube can be used to get an accurate platelet count if a patient’s platelets clump in the EDTA tube. The platelet count from the sodium citrate tube should be multiplied by 1.1 to account for the different blood to additive ratio in the citrate tube.
Hemolyzed specimens should not be processed since there could be activation of the clotting factors. Lipaemic or icteric specimens may also interfere with the instrumentation's optical system affecting the coagulation specimen result.
There have been recent articles indicating that drawing a discard tube is not necessary before drawing the sodium citrate tube. In the CLSI coagulation document (H21-A5) it is still recommended that the citrate tube is the second or third tube drawn. However, in the CLSI guideline for Venipuncture Collection (H3-A6) it states that for routine PT and APTT, the first tube drawn may be used for testing. It is also important to ensure a discard tube is drawn when a safety blood collection set is used and only a citrate tube is ordered, as the tubing may contain up to 0.5 mL of air that will be drawn into the tube and displace blood volume.
Greiner Bio-One blood collection tubes should be stored at 4-25°C (39-77°F).
VACUETTE® Tubes are irradiated to achieve sterility. These tubes are sterile on the interior only. We cannot recommend re-sterilizing the tubes, primarily due to pressure changes that take place during the re-sterilization cycle. Our tubes are under a specific negative pressure. During the re-sterilization cycle, this negative pressure may be disrupted and therefore the tubes may not draw the proper blood volume.
No, GBO does not claim that any of the tubes are endotoxin free.
|Tube Type||Recommended g-force relative centrifugal force (rcf)||Time (min)|
|VACUETTE ® Serum Tubes / with Sep / with Beads||1800 - 2200 g||10-15|
|VACUETTE ® EDTA Tubes / with Sep||1800 - 2200 g||10-15|
|VACUETTE ® Heparin Plasma Tubes / with Sep||1800 - 2200 g||10-15|
|VACUETTE ® Glucose Tubes||1800 - 2200 g||10-15|
|VACUETTE ® Homocystein Detection Tubes||2000 - 2200 g||10|
|VACUETTE ® Coagulation Tubes|
|- Platelet tests (PRP)||150 g||5|
|- Routine tests (PPP)||1500 - 2000 g||10|
|- Preparation for deep freeze plasma (PFP)||2500 - 3000 g||20|
VACUETTE® Blood Collection Tubes are made from a special formulation of the plastic, polyethylenetherephthalate (PET). This special formulation is extremely strong, dimensionally stable, and resistant to chemicals and breakage.
The expiry date indicates the shelf life of the product as determined by functional testing. To ensure accurate draw and test reliability, tubes should not be used after the expiry date. Expiry dates (last day of the month indicated) are printed on every VACUETTE® Blood Collection Tube and packaging.
Yes, VACUETTE® Blood Collection Tubes have a sterile interior.
Centrifuge the tubes at 1.800-2200g for 10-15 minutes. Please note that centrifuges need some time to accelerate the rotor to up to 2200g. This acceleration time should be in addition to the 10-15 minutes. The yield of serum is best at temperatures between 20-22°C. Centrifugation at refrigerated temperatures (4°C) may result in partial or no movement of the gel. Set the temperature between 20-22°C. Please note that tubes stored under refrigerated conditions should be allowed to reach room temperature before blood collection.