Greiner Bio-One has been manufacturing microplates for diagnostics and immunological research for over 30 years. with automated systems.

The microplate footprint is compatible with automated systems. A key step in ELISA is the binding of one assay component – antigen or antibody – to the solid surface by passive adsorption. Therefore, the features of this surface are crucial for the performance of the  assay. All ELISA microplates from Greiner Bio-One are made out of high-quality virgin polystyrene. The resin is highly transparent and therefore ideally  suited for optical measurements. Beside products made of clear polystyrene for colorimetric measurements, Greiner Bio-One offers a wide variety of black and white ELISA microplates for  luminescence and fluorescence measurements. The quality of our immunological products is constantly controlled in our quality assurance laboratory by means of ELISA.

Additional Product Information

ELISA (Enzyme Linked Immunosorbent Assay) is one of the most widely used biochemical methods in laboratory analysis and diagnostics. Analytes such as peptides, proteins, antibodies and hormones can be detected selectively and quantified in low concentrations among a multitude of other substances. Additionally, ELISAs are rapid, sensitive, cost effective and can be performed in a high-throughput manner. An ELISA is used in a vast variety of different types of assays (direct ELISA, indirect ELISA, sandwich ELISA, competitive ELISA). All ELISA variants are based on the same principle, the binding of one assay component – antigen or specific antibody – to a solid surface and the selective interaction between both assay components.

Greiner Bio-One manufactures a large variety of Immunology and ELISA Plates. Made of clear, black, or white polystyrene, the 96 well Immunology plates / 96 well ELISA plates are used for diagnostic and immunological research applications, maintain a high quality standard for consistency and reproducibility, and are available in two surface selections: 

High-Binding 96 Well Plates - The polystyrene surface has been specifically treated to provide a hydrophilic surface for increased protein binding.

Medium-Binding 96 Well Plates - The polystyrene surface is less hydrophilic than the surface of a high-binding microplate, and therefore tends to be more suitable for non polar proteins and peptides.

Since attachment to a solid surface based upon passive adsorption depends on properties of the molecule to be bound, it is advisable to compare performance of both high and medium binding products when developing a new assay.

Product list

Application Note: Influence of coating buffer and incubation conditions on ELISA performance
Application Notes / PDF, 810 KB
Application Note: Insulin ELISA on high binding MICROLON® 600 and CELLSTAR® microplates
Scientific Publications / PDF, 761 KB
Forum No 9: Microplates for Enzyme Linked Immunosorbent Assays (ELISA)
Scientific Publications / PDF, 1 MB
96 Well ELISA Microplates
Flyer / PDF, 909 KB
96 Well ELISA Strip Plates
Flyer / PDF, 693 KB
Sign up to our newsletter or request more information here