Poly-D-Lysine (PDL) & Poly-L-Lysine (PLL) are synthetic poly-peptides commonly used for cell culture coatings, providing a highly positive charged surface that enhances cell adhesion. PDL coatings are more durable based on their resistance against proteases released by certain cell types.
Collagen Type I (Col. Type I) is a major component of the extracellular matrix (ECM) of numerous cell types. Collagen coatings help mimic the in-vivo microenvironment and promote cell adhesion, proliferation and differentiation of mesenchymal and connective tissue-derived cells.
Fibronectin is a glycoprotein that connects membrane-spanning receptors (“integrins”) to extracellular matrix proteins like collagens and heparan sulfate proteoglycans. Fibronectin-binding integrin receptors are present in cell membranes of numerous cell types making fibronectin coatings a preferred choice to facilitate fast, strong attachment, spreading and growth of many cell types.
Laminin is a glycoprotein and major component of the basement membrane. It binds to integrin cell surface receptors in the cell membrane and to type IV collagen, fibronectin and proteoglycans in the extracellular matrix. As a vital support for scaffolding the basal lamina, Laminin coatings promote structural integrity, cell adhesion, migration and tissue organisation, and are commonly used for culturing neurons, muscle cells, epithelial and endothelial cells.
Basement membrane extract (BME) is derived from Engelbreth-Holm-Swarm mouse sarcoma, composed predominantly of laminin, type IV collagen and heparan sulfate proteoglycan. It mimics the basement membrane extracellular environment in many tissues and is used as a “thin coating” for cell adhesion and support of cell-specific phenotype in complex 2D-cell models.
