Clear microplates
- Usage: Colorimetric Measurements
Greiner Bio-One offers a comprehensive portfolio of microplates for high-throughput screening. The plates differ in format, design, base material, color, surface properties abd bottom configuration. Our microplates for high-throughput screening are available with 96, 384 or 1536 well, in the colors black, white and clear. The microplates are made of high-quality materials and are used in basic science, biotechnology and pharmaceutical drug development.
Are you looking for cell culture microplates? Here you can find our portfolio for cell-based HTS assays.
Polypropylene (PP) and polystyrene (PS) are the standard materials used to manufacture the majority of microplates.
Polystyrene is a highly clear polymer with excellent optical properties which makes it ideal for precise optical measurements. Polystyrene is also characterised by its ability to bind biomolecules, such as proteins, and it is therefore often used for manufacturing immunological products. Polystyrene is also suitable for work with cell cultures.
Polypropylene is characterised by its excellent chemical and thermal stability. It is the ideal polymer for storage of active reagents, patient samples or biomolecules in microplates.
In addition, Greiner Bio-One manufactures microplates with special requirement profiles, such as the UV-Star® or SCREENSTAR microplates made from different cycloolefins. They are characterised by their low level of autofluorescence, exceptionally high clarity, especially in the UV range, and greater chemical stability when compared with polystyrene.
µClear® and UV-Star® Film Bottom Microplates
The development of a new and patented processing technique has made it possible for us to produce microplates with ultra-thin films, without the use of adhesives or solvents – the µClear® and UV-Star® products. This special method eradicates the risk of leaking wells.
SensoPlate Glass Bottom Microplates
SensoPlate glass bottom microplates consist of a black pigmented polystyrene frame on to which a 175 μm thick borosilicate glass bottom is bonded. Thanks to the high optical quality of the glass bottom as well as the minimal bending, SensoPlate are especially recommended for fluorescence correlation spectroscopy and sophisticated microscopic applications.
Non-binding surfaces
Non-binding surfaces from Greiner Bio-One are characterised by their low binding capacity for biomolecules such as DNA, RNA, peptides and proteins. The repellent property of the non-binding surfaces for biomolecules can be advantageous in biochemical assays by increasing the sensitivity, reducing the background and improving the signal-to-noise ratio.
Achieved through a chemical modification of the resin rather than a resin mixture with potential to leach, the non-binding surface from Greiner Bio-One is stable under common assay conditions and does not degrade during short-term storage.
Lids for Microplates
Four different polystyrene lid designs are available:
Lids are available in two options, sterile and non-sterile. If microplates are supplied with lids, as in the case of CELLSTAR® products, the 96 well microplates always include lids with a high profile and the 384 well microplates always include plate lids with a low profile. In addition all products are also available without lids, which means that the type of lid can be selected as required.
Black pigmented microplates are commonly used for fluorescence applications, whereas white pigmented microplates typically support luminescence measurements, and are sometimes used to enhance fluorescence signal intensity. Both pigmentations help overcome critical issues for these techniques, such as background, autofluorescence, and well-to-well crosstalk. Pigmentation does not impact the material or surface chemistry, and black or white polystyrene microplates are available with different surface properties. Polypropylene microplates are as well available with black and white pigmentation and offer lower biomolecule binding and higher thermal and chemical resistance than polystyrene.
The µClear® microplates are clear bottom microplates with a thin polystyrene film bottom (190 µm +/- 20 µm for a 96 and 384 well formats and 75 µm +/- 10 µm for the 1536 well format).
Commonly µClear® microplates are used in cell based assays as they allow the microscopic monitoring of the cell growth. Additionally µlear® microplates have increased sensitivity down to 340 nm due to a reduction in background in transmission measurements.
Yes! For the determination of nucleic acid and protein concentrations at 260 nm or 280 nm without background interference the UVStar® microplates are an ideal alternative to expensive and fragile quartz glass microplates or cuvettes.
Different UVStar® microplates are available:
High binding" polystyrene surfaces are hydrophilised using a special method of physical treatment, which allows increased binding capacity for polar molecules. The binding of non-polar molecules is favored by the hydrophobic "Medium binding" polystyrene surface.
Microplates made out of polystyrene do not withstand the high temperatures of the autoclaving process (specified thermal stability of polystyrene = -20°C - + 60°C) Microplates made out of polypropylene do have a higher temperature tolerance (specified temperature range of polypropylene = -196°C to + 121°C).
All microplates are intended for single use only and autoclaving is not recommended as autoclaving can influence the tight physical dimensions and tolerances of the microplates.
However most of our microplates are as well available in a sterile version and autoclaving is not necessary.
96 well microplates are available with different well designs:
High throughput assays and screening are a mainstay of basic research, drug development, diagnostics and quality control. It’s also continuously developing to meet changing needs.
Assays and screening campaigns are becoming more sophisticated and information-rich. If this sounds relevant to your work, visit our HTS blog. Our articles are made to help you get the best results from your high throughput screening campaigns.
We empower people to make a difference in health. Please tell us how we can help you! We are looking forward to your request.
Contact / We are here to help you