CELLVIEW ZELLKULTUR SCHALE, PS, 35/10 MM,
GLASBODEN, 1 KOMPARTIMENT, ADVANCED TC, STERIL, 10 ST./BTL.
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Art. Nr.: 627965
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Preisangabe
Basisdaten
Beschreibung: |
CELLVIEW ZELLKULTUR SCHALE, PS, 35/10 MM, GLASBODEN, 1 KOMPARTIMENT, ADVANCED TC, STERIL, 10 ST./BTL. |
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Sterilität: | steril |
Stück / Karton: | 40 |
Stück / UVP: | 10 |
Verpackung
Verpackungsgewicht: | 0,40 kg |
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Verpackungsgrößen: | 195 x 142 x 122 mm |
Stück / Karton: | 40 |
Stück / UVP: | 10 |
PAL: | 22800 |
Detailinformationen
Füllvolumen (ml): | 0.00 |
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CELLview Zellkultur Schale mit Glasboden
- Frei von nachweisbarer DNase, RNase und humaner DNA
- Frei von nachweisbaren Endotoxinen, frei von zytotoxischen Substanzen
- Vorteile:
- Kompartimentierung ermöglicht Multiplex-Analysen
- Maximale Planarität durch eingebetteten Glasboden
- Zusätzliche TC-Oberflächenbehandlung und Advanced TC Oberflächenmodifikation erhältlich
- Anwendungen:
- Phasenkontrastaufnahmen
- Fluoreszenzmikroskopie
- Konfokale Mikroskopie
- Lebendzellanalyse
- Differenzielle Interferenz-Kontrast-Mikroskopie
- Polarisationsmikroskopie
- Fluoreszenz-In-Situ-Hybridisierung (FISH)
- Eigenschaften des Glasbodens:
- Hochtransparentes, farbloses Borsilikatglas der hydrolytischen Klasse 1 (DIN ISO 719)
- Glasdicke 175 µm +/- 15 µm
- Maximale spektrale Transmission; keine Autofluoreszenz
- Hervorragende Planarität
- Gefertigt nach ISO 8255-1:1986 (Optics and optical instruments - Microscopes - Cover glasses)
- Anzahl Kompartimente: 1
- Durchmesser: 35 mm; Höhe: 10 mm
- Wachstumsfläche: 8,7 cm²
- Max. Volumen: 10 ml
- Arbeitsvolumen: 5 ml
- Oberflächenbehandlung: Advanced TC
- Steril
Videos
Drug treatment during live cell imaging
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:
- Video 1 - control (no drugs added)
In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.
- Video 2 - Nocodazole added, final concentration 10 µM
Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.
- Video 3 - Latrunculin B added, final concentration 1 μM
Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi rearrangement was completed.
- Video 4 - Brefeldin A added, final concentration 5 μg/ml
Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.
Performing these experiments in parallel in CELLview dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes.
Zugehörige Downloads
IFU CELLdisc
IFU Gebrauchsanweisungen / PDF, 961 KB / 678101, 678104, 678108, 678116, 678140, 678904, 678908, 678916, 678940
IFU Gebrauchsanweisungen / PDF, 961 KB / 678101, 678104, 678108, 678116, 678140, 678904, 678908, 678916, 678940
Application Note: siRNA dependent gene silencing in HeLa cells cultivated on
Anwendungshinweise / PDF, 508 KB
Anwendungshinweise / PDF, 508 KB
Application Note: Cultivation and Differentiation of Human Adipose Derived Mesenchymal Stem Cells with CELLSTAR® and CELLCOAT® Cell Culture Products
Anwendungshinweise / PDF, 539 KB
Anwendungshinweise / PDF, 539 KB
Application Note: Improved Cultivation and Differentiation of Embryonic Stem Cells
Anwendungshinweise / PDF, 2 MB
Anwendungshinweise / PDF, 2 MB
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