CELLVIEW ZELLKULTUR SCHALE, PS, 35/10 MM,

GLASBODEN, 4 KOMPARTIMENTE, STERIL, 10 ST./BTL.
Art.nr.: 627871
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Basisdaten
Beschreibung: CELLVIEW ZELLKULTUR SCHALE, PS, 35/10 MM,
GLASBODEN, 4 KOMPARTIMENTE, STERIL,
10 ST./BTL.
Sterilität: steril
Stück/KAR: 40
Stück/UVP: 10
Verpackung
Gewicht/KAR: 0,40 kg
Kartonabmessung: 195 x 142 x 122 mm
Stück/KAR: 40
Stück/UVP: 10
PAL: 22400
Detailinformationen

CELLview Zellkultur Schale mit Glasboden

  • Frei von nachweisbarer DNase, RNase und humaner DNA
  • Frei von nachweisbaren Endotoxinen, frei von zytotoxischen Substanzen
  • Vorteile:
    • Kompartimentierung ermöglicht Multiplex-Analysen
    • Maximale Planarität durch eingebetteten Glasboden
    • Zusätzliche TC-Oberflächenbehandlung und Advanced TC™ Oberflächenmodifikation erhältlich
  • Anwendungen:
    • Phasenkontrastaufnahmen
    • Fluoreszenzmikroskopie
    • Konfokale Mikroskopie
    • Lebendzellanalyse
    • Differenzielle Interferenz-Kontrast-Mikroskopie
    • Polarisationsmikroskopie
    • Fluoreszenz-In-Situ-Hybridisierung (FISH)
  • Eigenschaften des Glasbodens:
    • Hochtransparentes, farbloses Borsilikatglas der hydrolytischen Klasse 1 (DIN ISO 719)
    • Glasdicke 175 µm +/- 15 µm
    • Maximale spektrale Transmission; keine Autofluoreszenz
    • Hervorragende Planarität
    • Gefertigt nach ISO 8255-1:1986 (Optics and optical instruments - Microscopes - Cover glasses)
  • Anzahl Kompartimente: 4
  • Durchmesser: 35 mm; Höhe: 10 mm
  • Wachstumsfläche: 1,9 cm²/Kompartiment
  • Max. Volumen: 1,5 ml/Kompartiment
  • Arbeitsvolumen: 0,1 ml für die Aussaat und Färbung nur auf der Glasfläche; 0,5 ml für die Zellkultivierung im gesamten Kompartiment
  • Steril

Stück pro Beutel/Karton: 10/40

Videos
Drug treatment during live cell imaging
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:
  • Video 1 - control (no drugs added)
    In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.

  • Video 2 - Nocodazole added, final concentration 10 µM
    Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.

  • Video 3 - Latrunculin B added, final concentration 1 μM
    Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi
    rearrangement was completed.

  • Video 4 - Brefeldin A added, final concentration 5 μg/ml
    Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.

Performing these experiments in parallel in CELLview™ dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes.

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