Buizen / bekers

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Greiner Bio-One International

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Buizen / bekers

Tubes are made of the following materials:

Polystyrene: Is ideally suited for optical measurements as a result of its high clarity.
Polypropylene: Displays high thermal, mechanical and chemical resistance. It is therefore recommended for the storage of chemical and biological samples.
Polyethylene: Is characterised by high thermal and chemical resistance.

The range of tubes available:

with round or conical bottom
with or without skirt
in sterile or non-sterile version

In addition, it is possible to order appropriate closures in the form of grip stoppers and screw caps.
The product range is completed by tubes with a two-position vent stopper. This special stopper enables ventilation or an airtight closure of the tube, depending on the position of the stopper.

Please refer to the Technical Appendix for information on the max. relative centrifugal force (RCF) and chemical and thermal resistance of our tubes.

Gerelateerde FAQs

How precise is the graduation of 15 ml and 50 ml-polypropylene tubes?

The graduation of 15 ml- (177 xxx) and 50 ml-polypropylene tubes is precise in a tolerance range of +/- 2%.

Are Greiner Bio-One polystyrene tubes autoclaveable?

Centrifuge tubes as well as other products of polystyrene are not autoclaveable. Polystyrene is resistant to temperatures between -20 °C and +60 °C. Polystyrene products will deform themselves because of the high temperature (+120 °C) during the autoclaving process.

Does Greiner Bio-One offer polycarbonat tubes for transfections?

Yes, the following polycarbonate tubes are available

Product Number	Dimensions	Volume	Additional Feature
160 501	16,0 x 100 mm	12 ml	round bottom
172 501	16,5 x 103 mm	13 ml	conical bottom
169 501	16,0 x 152 mm	20 ml	round bottom

We want to use the 15/50ml polypropylene tubes for highspeed centrifuging. Is it possible to use these tubes at a centrifugal force of 12.000G?

Maximal centrifugation forces depend on the type of rotor and are specified as follows:

Product Description	Product Number	max. RCF Fixed Angle Rotor [g]	max. RCF Swinging Bucket Rotor [g]
15ml PP tube	188xxx	15.000	4.000
50ml PP tube	210xxx	11.500	2.800
50ml PP tube	227xxx	9.500	3.200

Is there to the article Cat.-No.187262 (Tubes Round Bottom) also a rack?

Greiner Bio One does not offer a special rack for item 187262. It is recommended to use a standard tube rack for this product.

I use a lot of your 15ml polystyrene tubes and your 50ml polypropylene for rat cell culture. After washing, are they able to be thrown in the recycling waste?

All laboratories which manipulate potentially hazardous biological agents and materials, and generate waste containing such agents are responsible for the separation, packaging and treatment of their laboratory waste prior to its removal and disposal.

Greiner Bio One as supplier of disposable plastic ware cannot indicate the appropiate waste disposable the latter beeing dependent on the respective customer application and country-specific regulations. All Greiner plastic disposables are for single use only and cannot be recycled.

Please find below universal recommendations for biological waste disposal:

Biological waste includes:

liquids such as used cell culturing media, supernatant, blood or blood fractions (serum), etc., which contain viable biological agents;
materials considered pathological, including any part of the human body, tissues and bodily fluids, but excluding fluids, extracted teeth, hair, nail clippings and the like that are not infectious;
any part of an animal infected [or suspected to be infected] with a communicable disease;
non-sharp, solid laboratory waste (empty plastic cell culture flasks and petri dishes, empty plastic tubes, gloves, wrappers, absorbent tissues, etc.) which may be, or is known to be, contaminated with viable biological agents;
all sharp and pointed items used in medical care, diagnosis, and research, including the manipulation and care of laboratory animals, which should be considered potentially infectious;
laboratory glassware which is known or suspected to be contaminated with hazardous biological agents.

In addition to the general packaging requirements specific requirements for biological waste must be followed:

a) Liquids containing Biohazardous Agents

Collect liquids in leak-proof containers such as flasks or bottles.
Liquid waste containers designed to withstand autoclaving temperatures must be used when steam sterilization is utilized. To allow pressure equalization, they should not be sealed.

b) Solids Containing Biohazardous Agents

Non-sharp, solid laboratory waste (empty plastic cell culture flasks and petri dishes, empty plastic tubes, gloves, wrappers, absorbent tissues, etc.) which may be, or is known to be, contaminated with viable biological agents should be collected in autoclavable bags.

c) Sterilization and Disinfection

Autoclaving (steam sterilization) is the preferred (and generally regarded as the most reliable) method of sterilizing biological waste. Depending on the volume of waste to be sterilized, it may be necessary to extend the duration of exposure to high temperature steam under pressure.
Steam sterilization is generally not recommended for laboratory waste contaminated with or containing a combination of viable biological agents and significant amounts of hazardous chemical or radioactive materials.
Containers of liquid waste must be placed into an autoclavable tray or pan of sufficient capacity to contain all liquid in the event of vessel failure or breakage inside the autoclave chamber. Use extreme caution when handling autoclaved liquids since they are hot and may boil over.
Autoclavable bags of solid waste should be closed but not sealed airtight to allow steam penetration before they are placed into the autoclave chamber.

What is the max. RCF of the 14 ml PP-Tubes (Cat.No.187 261) to be centrifugalized?

The maximum centrifuge capacity of PP-tube Cat. No. 187 261 is:

Swinging bucket rotor max. 4800 RCF
Fixed-angle rotor max. 34000 RCF