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Magnetic 3D Cell Culturing

The core technology is the magnetization of cells with NanoShuttle-PL. The cells can be aggregated with magnetic forces, either by levitation or printing, to form structurally and biologically representative 3D models in vitro.

The advantages of magnetic cell culture include:

Mimicking native tissue environment
Rapid 3D model formation within hours
No specialized equipment, media, or artificial substrate
Easy to handle / no sample loss
Allows co-culture

With magnetized spheroids, solution addition and removal is made easy by using magnetic force to hold them in a stationary position during aspiration, thereby limiting spheroid loss. Spheroids can also be picked up and transferred between vessels using magnetic tools such as the MagPen.

NanoShuttle-PL consists of gold, iron oxide, and poly-L-lysine. NanoShuttle-PL magnetizes cells by electrostatically attaching to cell membranes during an overnight static incubation. Magnetized cells will appear peppered with dark nanoparticles after incubation. NanoShuttle-PL will stay attached to the cell membrane for up to 8 days, at which point it’s released into the 3D culture. NanoShuttle-PL is biocompatible, having no effect on metabolism, proliferation, and inflammatory stress, and even encouraging proliferation in 3D. Additionally, it does not interfere with experimental techniques, such as fluorescence, or Western blotting.