CELLVIEW CELL CULTURE DISH, PS, 35/10 MM,
GLASS BOTTOM, 1 COMPARTMENT, TC, STERILE, 10 PCS./BAG
Item No.: 627860
Product Downloads
Basic data
| Product description: |
CELLVIEW CELL CULTURE DISH, PS, 35/10 MM, GLASS BOTTOM, 1 COMPARTMENT, TC, STERILE, 10 PCS./BAG |
|---|---|
| Sterility: | sterile |
| Packing unit: | 40 |
| Quantity per inner pack: | 10 |
Packaging
| Packaging weight per CAR: | 0.34 kg |
|---|---|
| Carton dimension: | 195 x 145 x 125 mm |
| Packing unit: | 40 |
| Quantity per inner pack: | 10 |
| PAL: | 22800 |
Detailed information
| Filling volume (ml): | 0.00 |
|---|
CELLview - Cell Culture Dish with Glass Bottom
- Free of detectable DNase, RNase, human DNA
- Non-pyrogenic, non-cytotoxic
- Glass bottom features:
- High transparent achromatic borosilicate glass; hydrolytic class 1 (DIN ISO 719)
- Glass thickness 175 µm +/- 15 µm
- Maximal spectral transmission; no autofluorescence
- Advantages:
-Subdivided version enables simultaneous multiplex analysis
- Embedded glass bottom for maximal planarity
- Number of compartments: 1
- Diameter: 35 mm; height: 10 mm
- Growth area: 8.7 cm²
- Total volume: 10 ml
- Working volume: 5 ml
- Surface treatment: TC
- Sterile
Videos
Drug treatment during live cell imaging
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:
- Video 1 - control (no drugs added)
In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.
- Video 2 - Nocodazole added, final concentration 10 µM
Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.
- Video 3 - Latrunculin B added, final concentration 1 μM
Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi rearrangement was completed.
- Video 4 - Brefeldin A added, final concentration 5 μg/ml
Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.
Performing these experiments in parallel in CELLview dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes.
1 item added to your Cart
Would you like to continue shopping as a guest, or login / register as a customer?
Application Note: siRNA dependent gene silencing in HeLa cells cultivated on
Application Notes / PDF, 508 KB
Application Notes / PDF, 508 KB
Application Note: Cultivation and Differentiation of Human Adipose Derived Mesenchymal Stem Cells with CELLSTAR® and CELLCOAT® Cell Culture Products
Application Notes / PDF, 539 KB
Application Notes / PDF, 539 KB
Application Note: Selection of Cell Culture Surfaces for the Adipogenic Differentiation of Human Mesenchymal Stem Cells (hMSC)
Application Notes / PDF, 9 MB
Application Notes / PDF, 9 MB
Application Note: Improved Cultivation and Differentiation of Embryonic Stem Cells
Scientific Publications / PDF, 2 MB
Scientific Publications / PDF, 2 MB
It's the simplest way to know everything. We'd love to get in touch.