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Reliable cell adhesion and healthy cell growth
From complex ECM proteins to peptide-based coatings such as Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL): biologically functionalized cell culture surfaces provide the key prerequisites for stable cell attachment and physiologically relevant cell behavior. Protein and peptide coatings deliver essential signals from the cellular microenvironment and thereby promote cell adhesion, proliferation, and differentiation.
Pre-coated cell culture vessels – e.g., with ECM proteins such as collagen, laminin, and fibronectin as well as peptide-based coatings like PDL and PLL – offer a consistent, ready-to-use solution for sensitive primary cells, stem cells, and neuronal cells. Especially under serum-free or serum-reduced conditions, when natural adhesion factors are missing, a defined coating enables reproducible attachment and uniform growth.
Strong and homogeneous cell attachment reduces cell loss during washing steps and demanding applications such as transfection or differentiation. The result: more robust experiments, improved reproducibility, and more reliable assay performance. With the right coating, you can also achieve more physiologically relevant cell behavior – an important basis for robust data in research, development, and screening.
Key Facts
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Improved cell adhesion, cell spreading, and proliferation
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Supports demanding cell culture workflows including primary cells, stem cells, neuronal cells, and fastidious cell lines
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Suitable for serum-free and serum-reduced cell culture conditions
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Increased process reliability during washing steps, transfection, and differentiation
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Ready-to-use: quality-controlled coatings for consistent results
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Customized coatings as well as combined and dual coatings on request
Produits
Article:
628950
Boite de culture cellulaire, PS, 60x15mm, CELLCOAT®, Collagène type 1,20 PCS./sachet
Article:
655930
Microplaque culture cellulaire, 96 puits, PS, Fd F, puit cheminée, transparent, CELLCOAT®, Poly-L-Lysine, couvercle anneaux condensat°, 5 PCS./sachet
Article:
655936
Microplaque culture cellulaire, 96 puits, PS, Fd F, puit cheminée, µClear®, noir, CELLCOAT®, Poly-L-Lysine, couvercle anneaux condensat°, 5 PCS./sachet
Article:
655940
Microplaque culture cellulaire, 96 puits, PS, Fd F puit cheminée, transparent, CELLCOAT®, Poly-D-Lysinee, couvercle anneaux condensat°, 5 PCS./sachet
Article:
655944
Microplaque culture cellulaire, 96 puits, PS, Fd F, puit cheminée, µClear®, blanc, CELLCOAT®, Poly-D-Lysinee, couvercle anneaux condensat°, 5 PCS./sachet
Article:
655945
Microplaque culture cellulaire, 96 puits, PS, Fd F, puit cheminée, blanc, CELLCOAT®, Poly-D-Lysinee, couvercle anneaux condensat°, 5 PCS./sachet
Article:
655946
Microplaque culture cellulaire, 96 puits, PS, Fd F, puit cheminée, µClear®, noir, CELLCOAT®, Poly-D-Lysinee, couvercle anneaux condensat°, 5 PCS./sachet
Article:
655948
Microplaque culture cellulaire, 96 puits, PS, Fd F, puit cheminée, µClear®, noir, CELLCOAT®, Poly-D-Lysinee, couvercle anneaux condensat°, 20 PCS./sachet
Article:
655956
Microplaque culture cellulaire, 96 puits, PS, Fd F, puit cheminée, µClear®, noir, CELLCOAT®, Collagène type, couvercle anneaux condensat°, 5 PCS./sachet
Article:
657930
Plaque culture cellulaire multi puits, 6 puits, PS, transparent, CELLCOAT®, Poly-L-Lysine, couvercle anneaux condensat°, 5 PCS./sachet
Article:
657940
Plaque culture cellulaire multi puits, 6 puits, PS, transparent, CELLCOAT®, Poly-D-Lysinee, couvercle anneaux condensat°, 5 PCS./sachet
Article:
657950
Plaque culture cellulaire multi puits, 6 puits, PS, transparent, CELLCOAT®, Collagène type i, couvercle anneaux condensat°, 5 PCS./sachet
Article:
658910
Flacon culture cellulaire, 250ml, 75 cm², PS, CELLCOAT®, laminine, bch vissant filtre rouge, 5 PCS./sachet
Article:
658920
Flacon culture cellulaire, 250ml, 75 cm², PS, CELLCOAT®, fibronectine, bch vissant filtre rouge, 5 PCS./sachet
Documents associés
Application Note: siRNA dependent gene silencing in HeLa cells cultivated on
Notes d'application / PDF, 508 KB
Notes d'application / PDF, 508 KB
Application Note: Cultivation and Differentiation of Human Adipose Derived Mesenchymal Stem Cells with CELLSTAR® and CELLCOAT® Cell Culture Products
Notes d'application / PDF, 539 KB
Notes d'application / PDF, 539 KB
Application Note: Improved Cultivation and Differentiation of Embryonic Stem Cells
Publications scientifiques / PDF, 2 MB
Publications scientifiques / PDF, 2 MB
Application Note: Enhanced Transfection Efficiency on Protein Coated Microplates
Notes d'application / PDF, 451 KB
Notes d'application / PDF, 451 KB
Choose the optimal coating for your cell culture
Poly-D-Lysine (PDL) & Poly-L-Lysine (PLL)
Synthetic positively charged polymers that enhance electrostatic cell attachment – without intrinsic biological activity. Poly-lysine coatings are commonly used for neuronal cells, transfected cells, and fastidious cell lines. Poly-D-lysine is particularly resistant to enzymatic degradation and is therefore ideal for long-term cultures and demanding experimental workflows.
Collagen Type I
An important structural protein of the extracellular matrix (ECM) that supports cell attachment, cell spreading, and physiologically relevant cell behavior. Collagen type I is particularly suitable for primary cells and transfected cells – e.g., epithelial cells, fibroblasts, hepatocytes, and muscle cells – and is ideal for applications such as differentiation studies, cell–matrix interactions, and drug screening.
Laminin
A key component of the basement membrane with multiple binding domains for integrins and other ECM proteins. Laminin coatings promote adhesion, growth, and differentiation of sensitive cell types such as neuronal (stem) cells, epithelial cells, and endothelial cells. Particularly suitable for questions related to differentiation, migration, and chemotaxis.
Fibronectin
An ECM protein that supports cell adhesion and cell spreading via integrin binding sites. Fibronectin is frequently used for adhesion assays and is particularly helpful for cultures under serum-free or defined conditions, where natural adhesion factors are limited.
Looking for a tailored solution?
We offer flexible custom coatings – including combined and dual coatings – tailored to your cells, media conditions, and workflows. This gives you a defined, reproducible surface that fits your application.
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