TERASAKI PLATES: WITH 60 OR 72 WELLS
Greiner Bio-One Terasaki plates are suitable for all applications for serological determination of HLA antigens. The plates are supplied either with 60 or 72 wells, the plate dimensions stay the same.
The human leucocyte antigen (HLA) system is the major histo-compatibility complex (MHC) of humans and is composed of the two polymorphic classes HLA-I (A, B, and C) and HLA-II (DR, DQ, and DP). Basically, four different areas of indication can be distinguished for HLA typing: transplantation, transfusion, disease association and forensic.The serological determination of HLA proteins of the HLA-A, -B, -C and -DR genetic positions is primarily performed with the complement-dependent microlymphocytotoxicity test (LCT) or Terasaki test, which has been standardised since 1964. The basis for this test method is the cytolysis of the lymphocytes to be tested, which is caused by the antibody-antigen mediated activation of the complement system. Permeabilised lympho-cytes are generally stained with chromophores or fluorophores and evaluated microscopically.
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