Saliva Collection System
GBO Saliva Collection System (PDF, EN)
SAFETY Blood Collection Sets (PDF, EN)
VACUETTE® SECONDARY Tubes (PDF, EN)
VACUETTE® Safety Brochure (PDF, EN)
Early and Reliable Detection of Herpes Simplex Virus Type 1 and Varicella Zoster Virus DNAs in Oral Fluid of Patients With Idiopathic Peripheral Facial Nerve Palsy: Decision Support Regarding Antiviral Treatment? (PDF, EN)
United Forces Folder (PDF, EN)
Sample tubes can be delivered according to IATA / ADR/DRG transport regulations. They withstand 95kPa internal pressure.
When two saliva transfer tubes are used the specimen can be split at the time of collection and verified by the donor. The specimen can be split at time of collection using two saliva transfer tubes. When the first tube has been filled, simply remove it from the beaker and insert the second tube. Immediate sample splitting can be done in two or even more sample containers (tubes) which must be visibly clean and unused. The split samples are of identical quality.
During the rinsing step the drugs will be “trapped” in the solution. Once transferred to saliva transfer tubes the sample is stabilized by an additive in the tube.
Oral fluid specimens typically contain drugs and metabolites at considerably lower concentrations than in urine. Thresholds, or “cut-offs” for oral fluid are at least ten-fold lower than in urine and sensitive methods must be validated to enable reliable detection of recent drug use.
Comparability of the results is limited. The ratio saliva/blood differs from drug to drug. It depends on the protein binding and chemical property of each drug. Some drugs will be enriched in oral fluid compared to blood.
The laboratory can perform different tests and the combination of the results will give the added assurance that the specimen is valid for testing: total amount of sample (mL), % content of saliva, cortisol and salivary amylase. Cortisol and salivary amylase are endogenous substances secreted by different glands.
Oral fluid collection is done under gender-independent observation and without invasion of privacy. It should be ensured that the subject has not had anything to eat or drink for a minimum of 10 minutes before testing. As GBO saliva is liquid-based, even sample adulteration by pressing or sucking on the carrier material is not possible. Efforts to dilute the samples can be exposed by measuring different biomarkers.
It depends on the method used in the laboratory. Screening tests will give an overview on primary classes of drug representing the most common drugs like amphetamines, methamphetamines, opiates, cocaine, benzodiazepines and cannabis.
Oral fluid testing can detect drugs in the saliva roughly 30-60 minutes after ingestion. This short time frame makes it an excellent matrix to indicate if somebody might be under the influence of drugs. For urine testing it requires 2-6 hours to detect use after ingestion/use.
Almost all the drugs that can be found in blood can also be detected in oral fluid. Free drugs in blood differ from blood to oral fluid. By rinsing with the Saliva Extraction Solution the osmotic gradient between blood and oral fluid will be kept which boosts drugs’ diffusion. One exception is THC which is high protein-bounded. Detection of THC in oral fluid means the determination of oral contamination of THC smoking.
By rinsing the oral cavity with the yellow dyed saliva extraction solution (SES) the solution will be diluted with the near colourless oral fluid. This saliva SES mixture will be measured photometrically in a laboratory. The content of saliva within the sample will be calculated for the correct quantity determinationof analytes.
The solution consists entirely of food-grade materials. The Saliva Extraction Solution contains the food dye FD&C yellow n°5 (tartrazine). If any saliva extraction solution is swallowed, there is no health risk.
Occasionally an allergic reaction to FD&C yellow n°5 (tartrazine) may occur. Persons who do not tolerate aspirin and/or benzoic acid as well as asthmatics could be affected by this. Application is not recommended, if such cases of intolerance are known.
It is important to rinse for 2 minutes to collect enough sample for further analysis.